Friday, September 17, 2010

How Stem-Cell Treatment Saved A Police Dog

Almost crippled by arthritis, Dasty, a Chicago police dog, underwent stem-cell therapy and is back on the beat.
ANTONIO PEREZ/MCT
William HagemanChicago Tribune
CHICAGO—For a good half-hour, Dasty did the slobbery, happy dog thing—chomping on a hard rubber toy, offering it to visitors, chasing it when it was thrown. Hard to believe that just a few months ago, this 5-year-old German shepherd was virtually crippled by severe arthritis in his left rear leg.
"His quality of life was not good," says Dr. Cheryl Adams, a veterinarian at Arboretum View Animal Hospital in Downers Grove, Ill. "The poor guy could barely stand up."
Now he's as good as new, thanks to stem-cell treatment, a relatively new procedure in which Dasty's own fat cells provided the stem cells that were injected into the leg. It got him up and running again. And mobility is a prerequisite for Dasty's job. He's a member of the Chicago Police Department's narcotics unit.
Turns out, his arthritis was caused by Lyme disease.
"We don't know where he contracted it," says Officer Marion Anderson, a 21-year Chicago Police Department veteran who has been Dasty's partner for about four years. "He had been working for a couple of years, and one day I noticed he was getting up a little slow. Then it got slower. And he started limping."
Anderson didn't realize how bad it was until one night when Dasty, lying beside her, went to move and let out a yelp.
"You hear a dog cry out in pain, to hear that in the middle of the night scared the daylights out of me," she says.
Going up and down stairs became a problem, and getting in and out of a car was difficult. That's tough to see in any 3- or 4-year-old dog. But in a service dog . . .
"There was a point at his lowest when we thought he might have to be retired," says Officer Steve Martinez, who handles canine training for the narcotics division of the Chicago Police Department's organized crime division.
Anderson took Dasty to Arboretum View, which provides medical services for the dogs in the Chicago Police Department's canine unit. There his problem was diagnosed.
He received physical therapy, antibiotics and steroids, but the benefits were minor, and the side effects were not insignificant. His weight ballooned, and he developed, shall we say, bad eating habits.
"He started eating the handle off a purse, and he had to get stuff scoped out of his gut," Adams says. "Traditional treatments were just not working for him."
So Adams suggested the stem-cell treatment, which was developed by Vet-Stem, a California company that was founded in 2002 and in 2005 began working with several clinics in treating dogs with osteoarthritis and soft-tissue injuries. About 700 dogs have received the treatment.
In the procedure, a small incision is made in the abdomen—where fat cells are readily available—and some cells are removed (fat cells are used because 1 in 50 of them is a stem cell, the highest concentration in the body). The fat cells are shipped to Vet-Stem and processed. The stem-cell samples are returned—other samples are stored at Vet-Stem in case they're needed later—and administered.
In Dasty's case, it was both intravenously and through injection. That entire process takes just two days. The cost varies between $2,300 and $4,500, depending on the size of the dog and the number of injections needed, says Adams, who has treated between 40 and 50 patients. But the benefits are obvious.
"Stop it early, and they won't be crippled when they're 10," Adams says.
Dasty, who got a discount because he is a service dog, started treatment in March and was back at work within two weeks of that first incision. He had three treatments, and now he goes in periodically for checkups. He's on a low dose of steroids and is back to his original weight of 34 kilograms.
"It's such a heartwarming story," Anderson says, "that we were able to help out one of our service dogs when he does so much for the city."
How much?
"Right after one of his treatments, we were searching a cargo van, and he found $40,000," she says. "He's also found 21 kilos of cocaine, and a few months ago he found $173,000 in one search. He's a tremendous worker."

A Great Article On Aquarium Filtration & Maintenance From The Experts At Big Al's


BIG AL’S FISH TALK, FISHKEEPING 101 - PART 1
FILTRATION & MAINTENANCE
(See More Articles)

The first few weeks after setup Is about the most difficult (and therefore discouraging) period for the novice aquarist. Resist the temptation to load up your tank full of fish; rather, start with a few hardy fish (after the tank has been running at home for a day or two - never buy the tank and the fish on the same day!) and take well over a month to build up the population to the tank's capacity (usually one inch of fish per gallon, excluding tail) adding no more than a quarter of the maximum load every couple of weeks. Remember to allow room for growth. It is far better to understock a tank than to push it to its limit

Hardy Starter' fish include, mollies, swords, platies, larger tetras (i.e. black tetras serpaes, head and tail lights, NOT neons or cardinals!), danios and most barbs. Fish which should be added after the tank has been broken in include most bottom feeders and algae eaters, neons, cardinals, and angels. It is common for a new tank to go cloudy during the first week or two; this is caused by bacteria suspended in the water and will usually disappear within a few days to a week.

Mechanical filtration refers to the removal of particles from the water and chemical filtration uses specific media (activated carbon, zeolite, ion-exchange resins) to remove certain chemicals or chemical groups (fish do not only produce ammonia, it is just the most toxic at the top of a long list of organic by-products!). What is important to remember about chemical media is that they can only bond up so much (think of a sponge - it can only soak up so much water) and most are not rechargeable. Generally, chemical media should be replaced on a monthly basis.

Power filters which contain separate inserts (i.e. Aquaclears) should be cleaned in the following manner: Try to not deal with more than one insert within a few days to a week of another. Chemical media such as carbon and ammonia remover should be replaced every four weeks, but not both at the same time. The sponge insert (and this applies to canister filters such as Fluvals, too) rarely need to be replaced, but should be rinsed and squeezed out in a bucket of aquarium water when dirty, not under tap water, as the chlorine in the tap water will kill the majority of the beneficial bacteria living on it (sponges have the most surface are and, therefore, have the largest number of good bacteria living on them compared to the chemical inserts, so this is very important).

Another "big one" folks...neglect is all too common in our hobby and the root of many problems. Regular partial water changes are a MUST. There is no such thing as a perfect system where you never have to change water. There are many chemical compounds (nitrates, phosphates, organic acids) which are left behind and slowly build up, all of which are harmful to your fish. I recommend removing a minimum of 30% of your water every two to three weeks to keep this in check. Replacement water should come in slightly warmer (just compare tank temperature with your fingers) and be treated with a good quality water conditioner to neutralize chlorine and other harmful substances in tap water. If you have a home water purification unit, I do not advise relying on it unless it was very recently changed. Again, manufacturers can overrate the capacity of their products and fish gills are far more sensitive than our tastebuds (not to mention being the equivalent of our lungs!). A few things more: it is not necessary to At water sit when using a water conditioner (letting water sit out will bring it down to room temperature, adding much stress to a heated tropical tank). Unplug heaters and power filters when draining and allow heaters 10 minutes to readjust after filling before plugging in and rechecking their settings. Treat the new water only for the number of gallons you estimate you are changing; not for the entire tank capacity. You can overdose a little, the amount of chlorine added varies with the time of year (the most being spring and summer).

It is a very good idea to contact your local Public Works department (blue pages of your phone book) to find out whether your water is being treated with chloramine (a combination of chlorine and ammonia used when the treatment plant is pumping a long distance). There are special water conditioners for chloramine; if you are not sure, use them anyway

Use a Big Al's Gravel Cleaner to take the water out; this will remove most of the uneaten food and solid waste that accumulates in your substrate which is bad for the tank. Also, skim a little of the surface water off; the top layer is where carbon dioxide leaves the water and oxygen enters. This exchange is hindered by an accumulation of dust, oils and other organic compounds chemically attracted to the surface.

Use a Big Al's Deep Reach Scrub Brush to go over the sides of the tank, prior to drawing; this way, some of the algae wiped off will be removed with the water drained. By the way, the fish remain in the tank the whole time; taking them out will put far more stress on them and it is quite unnecessary.

NEVER confuse water lost to evaporation and then top up with a water change! The only thing that leaves a tank during evaporation is the water molecule itself (like distillation), all other chemicals simply remain in the tank and build up.

Finally, everyone has their own system for doing water changes. I am advocating a system which has worked successfully for me over many years, with a wide variety of fish species. Some people change very small amounts of their water (5 tolO%) often (weekly); but I find that the amounts taken out are insufficient to keep up with the chemical,waste buildup. Remember that if you are only removing 20%, 80% of the wastes are left behind. Others let their tanks go for months and then do a large cleanout, often losing a few fish in the process. The problems with this approach is that aver so much neglect, the water is so chemically different from the treated tap water that the water changes becomes a major stress, instead of the "breath of fresh air' it should be. The best way to handle such a scenario is to "freshen'' the tank water up by making several smaller 15 to 20% changes within a period of a week or so and then continue with larger, more regular changes. Whatever your system, vary it a little from time to time either by occasionally taking an extra 10 to 20% out andor increasing the frequency of the changes once in a while. This will go a long way towards keeping your chemical waste buildup under control.

Remember: the water can look clear and clean, but fish are swimming around in their own toilets (and we cannot see carbon monoxide or many other gases lethal to use either). Think about it.

CHRIS WHITELAW
Livestock Manager
Mississauga Location